Anti-Phospho-UBF (S484) UBTF Antibody

Product Name

Anti-Phospho-UBF (S484) UBTF Antibody

View all UBTF Antibodies

SKU/Catalog Number

A05134S484

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-UBF (S484) UBTF Antibody catalog # A05134S484. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-UBF (S484) UBTF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05134S484)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human UBF around the phosphorylation site of S484.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05134S484 is reactive to UBTF in Human, Mouse, Rat

Applications

A05134S484 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

89.406kDa

Background of UBTF

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

Jantzen H.M., Nature 344:830-836(1990).
Chan E.K.L., J. Exp. Med. 174:1239-1244(1991).
Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Immunohistochemistry validation of UBTF using Anti-Phospho-UBF (S484) UBTF Antibody (A05134S484).

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C

Gene/Protein Information For UBTF (Source: Uniprot.org, NCBI)

Alternative Names

90-KDa Nucleolus Organizer Region Autoantigen; Autoantigen NOR-90; NOR-90; nucleolar transcription factor 1; UBF; UBF1; UBF-1; UBF2; UBFUBF-1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1 UBTF CONDBA, NOR-90, UBF, UBF-1, UBF1, UBF2 upstream binding transcription factor nucleolar transcription factor 1|90-kDa nucleolus organizer region autoantigen|autoantigen NOR-90|upstream binding transcription factor, RNA polymerase I

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In this infographic, you will see the following information for UBTF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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