Anti-Phospholipase A1 member A PLA1A Antibody

Product Name

Anti-Phospholipase A1 member A PLA1A Antibody

View all PLA1A Antibodies

SKU/Catalog Number

A09234-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Phospholipase A1 member A PLA1A Antibody (Catalog # A09234-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Rat.

Storage & Handling

PLA1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Phospholipase A1 member A PLA1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09234-1)

Host

Rabbit

Contents

PLA1A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PLA1A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human PLA1A. The immunogen is located within amino acids 380 - 430 of PLA1A.

Cross-reactivity

At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms.

Reactive Species

A09234-1 is reactive to PLA1A in Human, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

49.7 kDa

Background of PLA1A

PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09234-1 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PLA1A antibody can be used for detection of PLA1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PLA1A

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