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Product Info Summary
| SKU: | M00029 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-PI 3 kinase p110 alpha Monoclonal Antibody
SKU/Catalog Number
M00029
BM4107 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PI 3 kinase p110 alpha Monoclonal Antibody catalog # M00029. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 kinase p110 alpha Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00029)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BEB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha
Reactive Species
M00029 is reactive to PIK3CA in Human, Mouse, Rat
Observed Molecular Weight
110 kDa
Calculated molecular weight
124.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00029 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Specific Publications For Anti-PI 3 kinase p110 alpha Monoclonal Antibody (M00029)
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3 Customer Q&As for Anti-PI 3 kinase p110 alpha Monoclonal Antibody
Question
We are currently using anti-PI 3 kinase p110 alpha Monoclonal antibody M00029 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The anti-PI 3 kinase p110 alpha Monoclonal antibody (M00029) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-23
Question
Is this M00029 anti-PI 3 kinase p110 alpha Monoclonal antibody reactive to the isotypes of PIK3CA?
J. Jones
Verified customer
Asked: 2015-05-12
Answer
The immunogen of M00029 anti-PI 3 kinase p110 alpha Monoclonal antibody is A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival,. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-12
Question
I was wanting to use your anti-PI 3 kinase p110 alpha Monoclonal antibody for WB for human adrenal tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adrenal tissue identification?
V. Lewis
Verified customer
Asked: 2014-11-17
Answer
It shows on the product datasheet, M00029 anti-PI 3 kinase p110 alpha Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adrenal tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-17
