Product Info Summary
SKU: | A14537-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PIG-Y Antibody
SKU/Catalog Number
A14537-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PIG-Y Antibody (Catalog # A14537-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PIG-Y Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14537-1)
Host
Rabbit
Contents
PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PIG-Y. The immunogen is located within the last 50 amino acids of PIG-Y.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody only detects isoform 1 of PIG-Y.
Reactive Species
A14537-1 is reactive to PIGY in Human, Mouse
Applications
A14537-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
8.058kDa
Background of PIGY
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PIG-Y antibody can be used for detection of PIG-Y by Western blot at 5 μg/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 μg/mL.
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Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 μg/mL.
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Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PIGY (Source: Uniprot.org, NCBI)
Gene Name
PIGY
Full Name
Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y
Weight
8.058kDa
Alternative Names
Phosphatidylinositol-glycan biosynthesis class Y protein; PIG-Y PIGY HPMRS6, PIG-Y phosphatidylinositol glycan anchor biosynthesis class Y phosphatidylinositol N-acetylglucosaminyltransferase subunit Y|phosphatidylinositol-glycan biosynthesis class Y protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIGY, check out the PIGY Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIGY: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIG-Y Antibody (A14537-1)
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No publications found for A14537-1
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Customer Reviews
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