Anti-PIGH Antibody

PIGH antibody

Boster Bio Anti-PIGH Antibody (Catalog # A12912). Tested in IHC applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A12912
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC

Product Name

Anti-PIGH Antibody

View all PIGH Antibodies

SKU/Catalog Number

A12912

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PIGH Antibody (Catalog # A12912). Tested in IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PIGH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12912)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human PIGH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12912 is reactive to PIGH in Human, Mouse

Applications

A12912 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.081kDa

Background of PIGH

Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.

Kamitani T., J. Biol. Chem. 268:20733-20736(1993).
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For PIGH (Source: Uniprot.org, NCBI)

Gene Name

PIGH

Full Name

Phosphatidylinositol N-acetylglucosaminyltransferase subunit H

Weight

21.081kDa

Superfamily

PIGH family

Alternative Names

EC 2.4.1.198; GPI-H; phosphatidylinositol glycan anchor biosynthesis, class H; phosphatidylinositol glycan, class H; phosphatidylinositol N-acetylglucosaminyltransferase subunit H; Phosphatidylinositol-glycan biosynthesis class H protein; phosphatidylinositol-glycan biosynthesis, class H protein; PIG-H PIGH GPI-H phosphatidylinositol glycan anchor biosynthesis class H phosphatidylinositol N-acetylglucosaminyltransferase subunit H|phosphatidylinositol-glycan biosynthesis, class H protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PIGH, check out the PIGH Infographic

PIGH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIGH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-PIGH Antibody

Question

Is this A12912 anti-PIGH antibody reactive to the isotypes of PIGH?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

The immunogen of A12912 anti-PIGH antibody is Synthesized peptide derived from C-terminal of human PIGH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-16

Question

Is a blocking peptide available for product anti-PIGH antibody (A12912)?

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

We do provide the blocking peptide for product anti-PIGH antibody (A12912). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-01

Question

I see that the anti-PIGH antibody A12912 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-03-06

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-03-06

Question

We are currently using anti-PIGH antibody A12912 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?

N. Li

Verified customer

Asked: 2019-02-08

Answer

The anti-PIGH antibody (A12912) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-08

Question

Does anti-PIGH antibody A12912 work on bovine IHC with placenta?

D. Wu

Verified customer

Asked: 2015-09-18

Answer

Our lab technicians have not tested anti-PIGH antibody A12912 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PIGH antibody A12912 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-09-18

Question

Will A12912 anti-PIGH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Walker

Verified customer

Asked: 2014-07-01

Answer

As indicated on the product datasheet, A12912 anti-PIGH antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-07-01

Order DetailsPrice
A12912-100uL

100uL

$360

Free Secondary Antibody

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A12912
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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