Click image to see more details
Product Info Summary
| SKU: | PA1680 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband®
SKU/Catalog Number
PA1680
BA3558 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody catalog # PA1680. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1680)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1680 is reactive to PIK3R2 in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
81.5 kDa
Background of PIK3R2
PIK3R2 (Phosphatidylinositol 3-kinase, regulatory subunit 2), also called p85-Beta, is an enzyme that in humans is encoded by the PIK3R2 gene. The PIK3R2 gene is mapped on 19p13.11. Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1680 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis tissue, 293T whole cell, HELA whole cell, mouse liver tissue, mouse brain tissue
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis tissue lysates,
Lane 2: 293T whole cell lysates,
Lane 3: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PA1680) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
Click image to see more details
IHC analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680).
PIK3R2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PIK3R2 Antibody (PA1680) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PA1680) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
Specific Publications For Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband® (PA1680)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband®
Question
Do you have a BSA free version of anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 available?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-06
Question
Has PA1680 been tested with mouse samples?
Verified customer
Asked: 2019-03-15
Answer
We have result image of the Anti-PI 3 Kinase P85 Beta/PIK3R2 Antibody (PA1680) on mouse samples for WB. However, we don't have a qualified IHC image with mouse samples.
Boster Scientific Support
Answered: 2019-03-18
Question
I see that the anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-09-25
Question
Is this PA1680 anti-PI 3 Kinase p85 beta/PIK3R2 antibody reactive to the isotypes of PIK3R2?
G. Anderson
Verified customer
Asked: 2017-10-24
Answer
The immunogen of PA1680 anti-PI 3 Kinase p85 beta/PIK3R2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2 (447-461aa KVYHQQYQDKSREYD), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-24
Question
Will anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 work on pig WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
Our lab technicians have not tested anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-20
