|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-PIK3R2/Pi 3 Kinase P85 Beta Antibody|
|Description||Rabbit IgG polyclonal antibody for Phosphatidylinositol 3-kinase regulatory subunit beta(PIK3R2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-PIK3R2/Pi 3 Kinase P85 Beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1680)|
|Replacement Item||This antibody may replace the following items: sc-85343|sc-85342 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2 (447-461aa KVYHQQYQDKSREYD), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Phosphatidylinositol 3-kinase regulatory subunit beta(PI3-kinase regulatory subunit beta/PI3K regulatory subunit beta/PtdIns-3-kinase regulatory subunit beta)|
|Molecular Weight||81545 MW|
|Protein Function||Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5- trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin- dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity). .|
|Sequence Similarities||Belongs to the PI3K p85 subunit family.|
|Alternative Names||Phosphatidylinositol 3-kinase regulatory subunit beta;PI3-kinase regulatory subunit beta;PI3K regulatory subunit beta;PtdIns-3-kinase regulatory subunit beta;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta;PI3-kinase subunit p85-beta;PtdIns-3-kinase regulatory subunit p85-beta;PIK3R2;|
|Research Areas|||signal transduction|signaling pathway|lipid signaling|lipid kinases| immunology|innate immunity|tlr signaling||
Background for Phosphatidylinositol 3-kinase regulatory subunit beta(PI3-kinase regulatory subunit beta/PI3K regulatory subunit beta/PtdIns-3-kinase regulatory subunit beta)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PIK3R2/Pi 3 Kinase P85 Beta Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PIK3R2/Pi 3 Kinase P85 Beta Antibody Images
Click the images to enlarge.
All lanes: Anti PIK3R2 (PA1680) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,