Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband™

Product Name

Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband™

View all PI 3-Kinase p85 beta Antibodies

SKU/Catalog Number

PB9777

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband™ catalog # PB9777. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9777)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9777 is reactive to PIK3R2 in Human

Applications

PB9777 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

81.545kDa

Background of PI 3-Kinase p85 beta

PIK3R2 (Phosphatidylinositol 3-kinase, regulatory subunit 2), also called p85-Beta, is an enzyme that in humans is encoded by the PIK3R2 gene. The PIK3R2 gene is mapped on 19p13.11. Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PB9777).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: 22RV1 Whole Cell Lysate,
Lane 3: MCF-7 Whole Cell Lysate,
Lane 4: SW620 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PB9777) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85 kDa. The expected band size for PIK3R2 is at 85 kDa.

Gene/Protein Information For PIK3R2 (Source: Uniprot.org, NCBI)

Alternative Names

P85B; p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta); PI 3Kinase p85 beta; PI 3-Kinase p85 beta; PI3K regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PIK3R2; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinas PIK3R2 MPPH, MPPH1, P85B, p85, p85-BETA phosphoinositide-3-kinase regulatory subunit 2 phosphatidylinositol 3-kinase regulatory subunit beta|PI3-kinase subunit p85-beta|PI3K regulatory subunit beta|phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)|phosphoinositide-3-kinase regulatory subunit beta|phosphoinositide-3-kinase, regulatory subunit 2 (beta)|ptdIns-3-kinase regulatory subunit p85-beta

For more info on PIK3R2, check out the PIK3R2 Infographic

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In this infographic, you will see the following information for PIK3R2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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