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Product Info Summary
| SKU: | M01184 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PIM1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01184
BM4395 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PIM1 Rabbit Monoclonal Antibody catalog # M01184. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01184)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ECB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PIM1
Reactive Species
M01184 is reactive to PIM1 in Human, Mouse
Observed Molecular Weight
34 kDa
Calculated molecular weight
35.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01184 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB:human k562 whole cell, human HEL whole cell, human RT4 whole cell, human HepG2 whole cell, human Caco-2 whole cell
IHC: human prostate carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of PIM1 using anti-PIM1 antibody (M01184).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human k562 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIM1 antigen affinity purified monoclonal antibody (M01184) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIM1 at approximately 34 kDa. The expected band size for PIM1 is at 45 kDa.
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Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PIM1 Antibody .
Specific Publications For Anti-PIM1 Rabbit Monoclonal Antibody (M01184)
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17 Customer Q&As for Anti-PIM1 Rabbit Monoclonal Antibody
Question
We have tried in the past anti-PIM1 Rabbit Monoclonal antibody for ICC on erythroleukemia a few months ago. I am using human, and We intend to use the antibody for IHC next. My question regards examining erythroleukemia as well as lower esophagus mucosa in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
I viewed the website and datasheets of our anti-PIM1 Rabbit Monoclonal antibody and I see that M01184 has been validated on human in both ICC and IHC. Thus M01184 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-13
Question
My colleagues were content with the WB result of your anti-PIM1 Rabbit Monoclonal antibody. However we have been able to see positive staining in erythroleukemia isoform 1: cytoplasm. nucleus. using this antibody. Is that expected? Could you tell me where is PIM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
According to literature, erythroleukemia does express PIM1. Generally PIM1 expresses in isoform 1: cytoplasm. nucleus., isoform 2: cell membrane. Regarding which tissues have PIM1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-23
Question
We are currently using anti-PIM1 Rabbit Monoclonal antibody M01184 for mouse tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The anti-PIM1 Rabbit Monoclonal antibody (M01184) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-13
Question
I am looking for to test anti-PIM1 Rabbit Monoclonal antibody M01184 on human erythroleukemia for research purposes, then I may be interested in using anti-PIM1 Rabbit Monoclonal antibody M01184 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
The products we sell, including anti-PIM1 Rabbit Monoclonal antibody M01184, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-19
Question
Is this M01184 anti-PIM1 Rabbit Monoclonal antibody reactive to the isotypes of PIM1?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The immunogen of M01184 anti-PIM1 Rabbit Monoclonal antibody is A synthesized peptide derived from human PIM1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-06
Question
We have seen staining in human kidney. What should we do? Is anti-PIM1 Rabbit Monoclonal antibody supposed to stain kidney positively?
K. Jha
Verified customer
Asked: 2019-11-05
Answer
From literature kidney does express PIM1. From Uniprot.org, PIM1 is expressed in lower esophagus mucosa, kidney, erythroleukemia, among other tissues. Regarding which tissues have PIM1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-05
Question
I am interested in using your anti-PIM1 Rabbit Monoclonal antibody for hyaluronan metabolic process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
We appreciate your inquiry. This M01184 anti-PIM1 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-26
Question
Can you help my question with product M01184, anti-PIM1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01184 anti-PIM1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-13
Question
Is there a BSA free version of anti-PIM1 Rabbit Monoclonal antibody M01184 available?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PIM1 Rabbit Monoclonal antibody M01184 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-11
Question
Would M01184 anti-PIM1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
As indicated on the product datasheet, M01184 anti-PIM1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-21
Question
Here is the WB image, lot number and protocol we used for erythroleukemia using anti-PIM1 Rabbit Monoclonal antibody M01184. Please let me know if you require anything else.
E. Anderson
Verified customer
Asked: 2018-07-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-09
Question
Would anti-PIM1 Rabbit Monoclonal antibody M01184 work on primate IF with lower esophagus mucosa?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
Our lab technicians have not tested anti-PIM1 Rabbit Monoclonal antibody M01184 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PIM1 Rabbit Monoclonal antibody M01184 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lower esophagus mucosa in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-08
Question
Is a blocking peptide available for product anti-PIM1 Rabbit Monoclonal antibody (M01184)?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
We do provide the blocking peptide for product anti-PIM1 Rabbit Monoclonal antibody (M01184). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-09-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-PIM1 Rabbit Monoclonal antibody M01184. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-04
Question
I was wanting to use your anti-PIM1 Rabbit Monoclonal antibody for IF for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
S. Singh
Verified customer
Asked: 2017-06-01
Answer
You can see on the product datasheet, M01184 anti-PIM1 Rabbit Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-01
Question
I see that the anti-PIM1 Rabbit Monoclonal antibody M01184 works with IF, what is the protocol used to produce the result images on the product page?
D. Mitchell
Verified customer
Asked: 2015-01-02
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-01-02
Question
Will anti-PIM1 Rabbit Monoclonal antibody M01184 work for IF with erythroleukemia?
C. Carter
Verified customer
Asked: 2013-11-26
Answer
According to the expression profile of erythroleukemia, PIM1 is highly expressed in erythroleukemia. So, it is likely that anti-PIM1 Rabbit Monoclonal antibody M01184 will work for IF with erythroleukemia.
Boster Scientific Support
Answered: 2013-11-26
