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Product Info Summary
| SKU: | M00201 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-PINK1 Antibody
SKU/Catalog Number
M00201
Size
50 µl
Description
Boster Bio Anti-PINK1 Antibody (Catalog # M00201). Tested in WB, IHC-P, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PINK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00201)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
38CT20.8.5
Isotype
IgG1
Immunogen
Recombinant PINK1 protein was used to produced this monoclonal antibody.
Reactive Species
M00201 is reactive to PINK1 in Human
Calculated molecular weight
62.8 kDa
Background of PINK1
This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00201 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Fluorescent image of PC12 cells stained with Pink1 (115-213) Antibody. M00201 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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All lanes : Anti-Pink1(115-213) at 1:1000 dilution
Lane 1: HeLa whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 63 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1(115-213). M00201 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. heart section using Pink1(115-213). M00201 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Specific Publications For Anti-PINK1 Antibody (M00201)
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