Click image to see more details
Product Info Summary
| SKU: | A02993 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Pituitary homeobox 1 Pitx1 Antibody
SKU/Catalog Number
A02993
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Pituitary homeobox 1 Pitx1 Antibody catalog # A02993. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pituitary homeobox 1 Pitx1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02993)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PITX1. AA range:121-170
Reactive Species
A02993 is reactive to PITX1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
34.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02993 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of Jurkat cells using Pitx1 Polyclonal antibody.
Click image to see more details
Western Blot (WB) analysis of specific cells using Pitx1 Polyclonal antibody.
Click image to see more details
Western blot analysis of lysates from Jurkat and COLO cells, using PITX1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Pituitary homeobox 1 Pitx1 Antibody (A02993)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Pituitary homeobox 1 Pitx1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Pituitary homeobox 1 Pitx1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Pituitary homeobox 1 Pitx1 Antibody
Question
Is this A02993 anti-Pitx1 antibody reactive to the isotypes of PITX1?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The immunogen of A02993 anti-Pitx1 antibody is Synthesized peptide derived from the Internal region of human Pitx1. at AA rangle: 90-170. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-26
Question
We are currently using anti-Pitx1 antibody A02993 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Pitx1 antibody (A02993) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
Question
I see that the anti-Pitx1 antibody A02993 works with WB, what is the protocol used to produce the result images on the product page?
J. Taylor
Verified customer
Asked: 2019-02-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-02-26
Question
Can you help my question with product A02993, anti-Pitx1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02993 anti-Pitx1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-02
Question
I would like to test anti-Pitx1 antibody A02993 on rat lower esophagus mucosa for research purposes, then I may be interested in using anti-Pitx1 antibody A02993 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The products we sell, including anti-Pitx1 antibody A02993, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-09
Question
Is a blocking peptide available for product anti-Pitx1 antibody (A02993)?
D. Patel
Verified customer
Asked: 2016-12-23
Answer
We do provide the blocking peptide for product anti-Pitx1 antibody (A02993). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-12-23
Question
I have attached the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Pitx1 antibody A02993. Please let me know if you require anything else.
E. Roberts
Verified customer
Asked: 2015-01-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-06
