Anti-Pituitary homeobox 1 Pitx1 Antibody

Boster Bio Anti-Pituitary homeobox 1 Pitx1 Antibody catalog # A02993. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02993
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-Pituitary homeobox 1 Pitx1 Antibody

See all PITX1 products

SKU/Catalog Number

A02993

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Pituitary homeobox 1 Pitx1 Antibody catalog # A02993. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pituitary homeobox 1 Pitx1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02993)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Pitx1. at AA range: 90-170

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02993 is reactive to PITX1 in Human, Mouse, Rat

Applications

A02993 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

Gene/Protein Information For PITX1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P78337

Gene ID

5307

Gene Name

PITX1

Full Name

Pituitary homeobox 1

Weight

34.128kDa

Superfamily

paired homeobox family

Alternative Names

BFTpituitary homeobox 1; CCF; Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; paired-like homeodomain 1; pituitary otx-related factor; POTXpituitary homeo box 1; PTX1Paired-like homeodomain transcription factor 1hindlimb expressed homeobox protein backfoot PITX1 BFT, CCF, LBNBG, POTX, PTX1 paired like homeodomain 1 pituitary homeobox 1|hindlimb expressed homeobox protein backfoot|homeobox protein PITX1|paired-like homeodomain transcription factor 1|pituitary homeo box 1|pituitary otx-related factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PITX1, check out the PITX1 Infographic

PITX1 infographic

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In this infographic you will see the following information for PITX1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-Pituitary homeobox 1 Pitx1 Antibody

Question

Is this A02993 anti-Pitx1 antibody reactive to the isotypes of PITX1?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

The immunogen of A02993 anti-Pitx1 antibody is Synthesized peptide derived from the Internal region of human Pitx1. at AA rangle: 90-170. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-26

Question

We are currently using anti-Pitx1 antibody A02993 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

The anti-Pitx1 antibody (A02993) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-22

Question

I see that the anti-Pitx1 antibody A02993 works with WB, what is the protocol used to produce the result images on the product page?

J. Taylor

Verified customer

Asked: 2019-02-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-26

Question

Can you help my question with product A02993, anti-Pitx1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-11-02

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02993 anti-Pitx1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-11-02

Question

I would like to test anti-Pitx1 antibody A02993 on rat lower esophagus mucosa for research purposes, then I may be interested in using anti-Pitx1 antibody A02993 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-07-09

Answer

The products we sell, including anti-Pitx1 antibody A02993, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-07-09

Question

Is a blocking peptide available for product anti-Pitx1 antibody (A02993)?

D. Patel

Verified customer

Asked: 2016-12-23

Answer

We do provide the blocking peptide for product anti-Pitx1 antibody (A02993). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-12-23

Question

I have attached the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Pitx1 antibody A02993. Please let me know if you require anything else.

E. Roberts

Verified customer

Asked: 2015-01-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-01-06

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A02993
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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