Anti-Pituitary homeobox 1 Pitx1 Antibody

Product Name

Anti-Pituitary homeobox 1 Pitx1 Antibody

View all PITX1 Antibodies

SKU/Catalog Number

A02993

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Pituitary homeobox 1 Pitx1 Antibody catalog # A02993. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pituitary homeobox 1 Pitx1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02993)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Pitx1. at AA range: 90-170

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02993 is reactive to PITX1 in Human, Mouse, Rat

Applications

A02993 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

34.128kDa

Background of PITX1

Anti-Cul2 antibody can be used in western blotting, ELISA and IP. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

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Western Blot (WB) analysis of Jurkat cells using Pitx1 Polyclonal antibody.

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Western Blot (WB) analysis of specific cells using Pitx1 Polyclonal antibody.

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Western blot analysis of lysates from Jurkat and COLO cells, using PITX1 Antibody. The lane on the right is blocked with the synthesized peptide.

Gene/Protein Information For PITX1 (Source: Uniprot.org, NCBI)

Alternative Names

BFTpituitary homeobox 1; CCF; Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; paired-like homeodomain 1; pituitary otx-related factor; POTXpituitary homeo box 1; PTX1Paired-like homeodomain transcription factor 1hindlimb expressed homeobox protein backfoot PITX1 BFT, CCF, LBNBG, POTX, PTX1 paired like homeodomain 1 pituitary homeobox 1|hindlimb expressed homeobox protein backfoot|homeobox protein PITX1|paired-like homeodomain transcription factor 1|pituitary homeo box 1|pituitary otx-related factor

For more info on PITX1, check out the PITX1 Infographic

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In this infographic, you will see the following information for PITX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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