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- Table of Contents
PJA1 antibody (A11179) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of PJA1 in mouse brain tissue with PJA1 antibody at 20 μg/ml.
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Anti-PJA1 Antibody
PJA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 – 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PJA1 Antibody
View all PJA1 Antibodies
View all PJA1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
PJA1 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
PJA1 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1. The immunogen is located within amino acids 50 - 100 of PJA1.
Cross-Reactivity
PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
71.0 kDa
Background of PJA1
Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).
Cite This Product
Anti-PJA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11179)
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Product Promise
We promise Anti-PJA1 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A11179
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026