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Product Info Summary
| SKU: | A11179 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PJA1 Antibody
SKU/Catalog Number
A11179
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PJA1 Antibody (Catalog # A11179). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PJA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-PJA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11179)
Host
Rabbit
Contents
PJA1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1. The immunogen is located within amino acids 50 - 100 of PJA1.
Cross-reactivity
PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.
Reactive Species
A11179 is reactive to PJA1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
71.0 kDa
Background of PJA1
Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11179 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 – 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PJA1 in human brain tissue lysate with PJA1 antibody at (A) 0.25 and (B) 0.5 μg/ml.
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Immunohistochemistry of PJA1 in mouse brain tissue with PJA1 antibody at 5 μg/ml.
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Immunofluorescence of PJA1 in mouse brain tissue with PJA1 antibody at 20 μg/ml.
Specific Publications For Anti-PJA1 Antibody (A11179)
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