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Product Info Summary
| SKU: | A11179 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PJA1 Antibody
SKU/Catalog Number
A11179
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PJA1 Antibody (Catalog # A11179). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PJA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-PJA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11179)
Host
Rabbit
Contents
PJA1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1. The immunogen is located within amino acids 50 - 100 of PJA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.
Reactive Species
A11179 is reactive to Pja1 in Human, Mouse, Rat
Applications
A11179 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
63.906kDa
Background of RNF70
Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 – 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PJA1 in human brain tissue lysate with PJA1 antibody at (A) 0.25 and (B) 0.5 μg/ml.
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Immunohistochemistry of PJA1 in mouse brain tissue with PJA1 antibody at 5 μg/ml.
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Immunofluorescence of PJA1 in mouse brain tissue with PJA1 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For Pja1 (Source: Uniprot.org, NCBI)
Gene Name
Pja1
Full Name
E3 ubiquitin-protein ligase Praja-1
Weight
63.906kDa
Alternative Names
EC 6.3.2; EC 6.3.2.-; FLJ11830; praja 1; praja ring finger 1; PRAJA1; RING finger protein 70; RNF70E3 ubiquitin-protein ligase Praja-1 Pja1|praja-1|praja ring finger ubiquitin ligase 1|E3 ubiquitin-protein ligase Praja-1|RING-type E3 ubiquitin transferase Praja-1|praja ring finger 1, E3 ubiquitin protein ligase|praja1, RING-H2 motif containing
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pja1, check out the Pja1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pja1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PJA1 Antibody (A11179)
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Customer Reviews
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