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Product Info Summary
| SKU: | A08677 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PJA2/Praja2 Antibody
SKU/Catalog Number
A08677
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PJA2/Praja2 Antibody catalog # A08677. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PJA2/Praja2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08677)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PJA2. AA range:141-190
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08677 is reactive to PJA2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
78.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08677 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Jurkat cells using PJA2 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using PJA2 Polyclonal antibody.
Specific Publications For Anti-PJA2/Praja2 Antibody (A08677)
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Customer Q&As
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7 Customer Q&As for Anti-PJA2/Praja2 Antibody
Question
Is this A08677 anti-PJA2/Praja2 antibody reactive to the isotypes of PJA2?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The immunogen of A08677 anti-PJA2/Praja2 antibody is Synthesized peptide derived from the Internal region of human PJA2. at AA rangle: 110-190. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-30
Question
See below the WB image, lot number and protocol we used for brain using anti-PJA2/Praja2 antibody A08677. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-17
Question
Does A08677 anti-PJA2/Praja2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can see on the product datasheet, A08677 anti-PJA2/Praja2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-16
Question
Would anti-PJA2/Praja2 antibody A08677 work on horse WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Our lab technicians have not validated anti-PJA2/Praja2 antibody A08677 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PJA2/Praja2 antibody A08677 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
My question regards to test anti-PJA2/Praja2 antibody A08677 on human brain for research purposes, then I may be interested in using anti-PJA2/Praja2 antibody A08677 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Johnson
Verified customer
Asked: 2018-03-05
Answer
The products we sell, including anti-PJA2/Praja2 antibody A08677, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-05
Question
We are currently using anti-PJA2/Praja2 antibody A08677 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
The anti-PJA2/Praja2 antibody (A08677) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-03
Question
I see that the anti-PJA2/Praja2 antibody A08677 works with WB, what is the protocol used to produce the result images on the product page?
R. Moore
Verified customer
Asked: 2013-06-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-06-07
