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Product Info Summary
| SKU: | A00743T638 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-PKC Alpha (phospho-T638) PRKCA Antibody
SKU/Catalog Number
A00743T638
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PKC Alpha (phospho-T638) PRKCA Antibody catalog # A00743T638. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC Alpha (phospho-T638) PRKCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00743T638)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human PKC α around the phosphorylation site of Threonine 638.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00743T638 is reactive to PRKCA in Human, Mouse, Rat
Calculated molecular weight
76.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00743T638 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:10-1:100
Validation Images & Assay Conditions
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Western blot (WB) analysis of PKC α (phospho-T638) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane5:MCF-7 whole cell lysate
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Immunohistochemistry (IHC) analyzes of p-PKC α (T638) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunoprecipitation of L02 cell lysate using PKC α (phospho-T638) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using SHP-2 (E574) pAb.“↑”（supernatant）; “↓”（deposition）
Specific Publications For Anti-PKC Alpha (phospho-T638) PRKCA Antibody (A00743T638)
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