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Product Info Summary
| SKU: | M01151 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKC epsilon Monoclonal Antibody
SKU/Catalog Number
M01151
BM5249 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKC epsilon Monoclonal Antibody catalog # M01151. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC epsilon Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01151)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACDA-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKC epsilon This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Reactive Species
M01151 is reactive to PRKCE in Human, Mouse, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
83.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01151 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of PKC epsilon expression in Jurkat cell lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Specific Publications For Anti-PKC epsilon Monoclonal Antibody (M01151)
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6 Customer Q&As for Anti-PKC epsilon Monoclonal Antibody
Question
I am interested in to test anti-PKC epsilon Monoclonal antibody M01151 on rat cervix carcinoma for research purposes, then I may be interested in using anti-PKC epsilon Monoclonal antibody M01151 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The products we sell, including anti-PKC epsilon Monoclonal antibody M01151, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-31
Question
My question regarding product M01151, anti-PKC epsilon Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01151 anti-PKC epsilon Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-06
Question
Is there a BSA free version of anti-PKC epsilon Monoclonal antibody M01151 available?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PKC epsilon Monoclonal antibody M01151 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-04
Question
Is this M01151 anti-PKC epsilon Monoclonal antibody reactive to the isotypes of PRKCE?
E. Anderson
Verified customer
Asked: 2018-05-23
Answer
The immunogen of M01151 anti-PKC epsilon Monoclonal antibody is A synthesized peptide derived from human PKC epsilon This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-23
Question
I see that the anti-PKC epsilon Monoclonal antibody M01151 works with WB, what is the protocol used to produce the result images on the product page?
T. Thomas
Verified customer
Asked: 2018-02-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-02-13
Question
Is a blocking peptide available for product anti-PKC epsilon Monoclonal antibody (M01151)?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
We do provide the blocking peptide for product anti-PKC epsilon Monoclonal antibody (M01151). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-12-13
