Anti-PKM2 Rabbit Monoclonal Antibody

Product Name

Anti-PKM2 Rabbit Monoclonal Antibody

View all PKM Antibodies

SKU/Catalog Number

M01173

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PKM2 Rabbit Monoclonal Antibody catalog # M01173. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKM2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01173)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GCB-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PKM2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01173 is reactive to PKM in Human, Mouse, Rat

Applications

M01173 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

190 kDa

Calculated molecular weight

57.937kDa

Background of PKM

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of PKM2 expression in HeLa cell lysate.

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Immunohistochemical analysis of paraffin-embedded human cervix cancer, using PKM2 Antibody.

Gene/Protein Information For PKM (Source: Uniprot.org, NCBI)

Alternative Names

CTHBP; Cytosolic thyroid hormone-binding protein; MGC3932; OIP3; OIP-3; OIP3EC 2.7.1.40; Opa-interacting protein 3; p58; PK, muscle type; PK2; PK3; PK3PKM; PKM2; Pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; pyruvate kinase, muscle; TCB; THBP1; THBP1p58; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; Tumor M2-PK PKM CTHBP, HEL-S-30, OIP3, PK32, TCB, THBP1, p58, PKM pyruvate kinase M1/2 pyruvate kinase PKM|OPA-interacting protein 3|PK, muscle type|cytosolic thyroid hormone-binding protein|epididymis secretory protein Li 30|pyruvate kinase 2/3|pyruvate kinase isozymes M1/M2|pyruvate kinase muscle isozyme|pyruvate kinase, muscle|thyroid hormone-binding protein 1|thyroid hormone-binding protein, cytosolic|tumor M2-PK

For more info on PKM, check out the PKM Infographic

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In this infographic, you will see the following information for PKM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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