Anti-PLA2G4A (Phospho-S505) Antibody (Monoclonal, 32P85)

Product Name

Anti-PLA2G4A (Phospho-S505) Antibody (Monoclonal, 32P85)

View all PLA2G4A Antibodies

SKU/Catalog Number

M00854

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-PLA2G4A (Phospho-S505) Antibody (Monoclonal, 32P85) catalog # M00854. Tested in WB, DotBlot applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PLA2G4A (Phospho-S505) Antibody (Monoclonal, 32P85) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00854)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

32P85

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser505 of human cPLA2 aa 493-538.

Reactive Species

M00854 is reactive to PLA2G4A in Human, Mouse

Calculated molecular weight

85.2 kDa

Background of PLA2G4A

PLA2G4A (Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al. (1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al. (1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands (Skorecki, 1995).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00854 is guaranteed for Dot blot, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information PLA2G4A

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