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- Table of Contents
PLA2G4A · Western blot design guide
PLA2G4A Western Blot Planning Guide
Plan a PLA2G4A Western blot around the catalog-observed 85.2 kDa band, image-backed PA2047 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PLA2G4A Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 85.2 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Parathyroid gland | |
| Negative control | Ovary |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 85.2 kDa |
| ⓘ | Localization | Cytoplasm / Golgi apparatus membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PLA2G4A Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Parathyroid gland |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA2047 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PLA2G4A Western Blot Band Size?
Use the product-observed 85.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 85.2 kDa | Matches the authoritative product WB observation. |
| 85.2 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PLA2G4A appearancePlan around 85.2 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 85.2 kDa; use this as the primary experimental expectation. |
| Calculated mass | 85.2 kDa from UniProt P47712; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA2047. |
| Situation | Likely cause | Next action |
|---|---|---|
| 85.2 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PLA2G4A band
PA2047 · PLA2G4A
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PLA2G4A Western blot
🧪Use Parathyroid gland as the first positive-control candidate and Ovary as the HPA Not detected negative candidate.
Positive control: Parathyroid gland (High)
Negative control: Ovary (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PLA2G4A
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Parathyroid gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Seminal vesicle | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adipose tissue | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced PLA2G4A Western Blot Tips
Deeper troubleshooting and optimisation questions for PLA2G4A, answered from its protein features.
Which band should guide the blot?
Use 85.2 kDa, the observation attached to the authoritative PA2047 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 85.2 kDa expectation.
Which positive control should I start with?
Start with Parathyroid gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Ovary as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PLA2G4A Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA2047 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PLA2G4A Western Blot Reagents
Human/Mouse/Rat-reactive PLA2G4A Western blot reagents with authoritative product imagery.
Real WB data
Anti-Phospholipase A2/PLA2G4A Antibody Picoband®
Cat # PA2047
Real WB data
Anti-Phospholipase A2/PLA2G4A Antibody Picoband®
Cat # PB9778
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P47712
- Human Protein Atlas — PLA2G4A
- PA2047 product record
- PMC12234970 — Multi-omics analysis of the effects of pla2g4a on the prognosis of various cancers and its experimental validation in breast cancer cell lines (Discover oncology, 2025)
- PMC4330957 — American ginseng regulates gene expression to protect against premature ovarian failure in rats (BioMed research international, 2015)
- PMC4372093 — Group IVA phospholipase A2 regulates testosterone biosynthesis by murine Leydig cells and is required for timely sexual maturation (The Biochemical journal, 2011)
