Anti-PLA2G6 Antibody Picoband™

Product Name

Anti-PLA2G6 Antibody Picoband™

View all PLA2G6 Antibodies

SKU/Catalog Number

A01648-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PLA2G6 Antibody Picoband™ catalog # A01648-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PLA2G6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01648-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PLA2G6 recombinant protein (Position: W715-L802).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01648-1 is reactive to PLA2G6 in Human, Mouse, Rat

Applications

A01648-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

89.903kDa

Background of PLA2G6

85 kDa calcium-independent phospholipase A2 is an enzyme that in humans is encoded by the PLA2G6 gene. The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PLA2G6 using anti-PLA2G6 antibody (A01648-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G6 antigen affinity purified polyclonal antibody (Catalog # A01648-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G6 at approximately 90KD. The expected band size for PLA2G6 is at 90KD.

Gene/Protein Information For PLA2G6 (Source: Uniprot.org, NCBI)

Alternative Names

calcium-independent phospholipase A2; EC 3.1.1.4; Group VI phospholipase A2; IPLA2; iPLA285 kDa calcium-independent phospholipase A2; IPLA2-VIA; NBIA2A; NBIA2B; PARK14GVI; patatin-like phospholipase domain containing 9; phospholipase A2, group VI (cytosolic, calcium-independent); PNPLA9 PLA2G6 CaI-PLA2, GVI, INAD1, IPLA2-VIA, NBIA2, NBIA2A, NBIA2B, PARK14, PLA2, PNPLA9, iPLA2, iPLA2beta phospholipase A2 group VI 85/88 kDa calcium-independent phospholipase A2|2-lysophosphatidylcholine acylhydrolase|85 kDa calcium-independent phospholipase A2|GVI PLA2|iPLA2-beta|intracellular membrane-associated calcium-independent phospholipase A2 beta|neurodegeneration with brain iron accumulation 2|palmitoyl-CoA hydrolase|patatin-like phospholipase domain-containing protein 9|phospholipase A2, group VI (cytosolic, calcium-independent)

For more info on PLA2G6, check out the PLA2G6 Infographic

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In this infographic, you will see the following information for PLA2G6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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