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Product Info Summary
| SKU: | A01648-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PLA2G6 Antibody Picoband®
SKU/Catalog Number
A01648-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PLA2G6 Antibody Picoband® catalog # A01648-1. Tested in ELISA, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLA2G6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01648-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PLA2G6 recombinant protein (Position: W715-L802).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01648-1 is reactive to PLA2G6 in Human, Mouse, Rat
Observed Molecular Weight
84 kDa
Calculated molecular weight
89.9 kDa
Background of PLA2G6
85 kDa calcium-independent phospholipase A2 is an enzyme that in humans is encoded by the PLA2G6 gene. The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01648-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEL whole cell, human Jurkat whole cell, human 293T whole cell, human K562 whole cell, rat testis tissue, mouse testis tissue
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Western blot analysis of PLA2G6 using anti-PLA2G6 antibody (A01648-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G6 antigen affinity purified polyclonal antibody (A01648-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLA2G6 at approximately 84 kDa. The expected band size for PLA2G6 is at 90 kDa.
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IF analysis of PLA2G6 using anti-PLA2G6 antibody (A01648-1).
PLA2G6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLA2G6 Antibody (A01648-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-PLA2G6 Antibody Picoband® (A01648-1)
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Customer Q&As
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7 Customer Q&As for Anti-PLA2G6 Antibody Picoband®
Question
Will anti-PLA2G6 antibody A01648-1 work for ELISA with testis?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
According to the expression profile of testis, PLA2G6 is highly expressed in testis. So, it is likely that anti-PLA2G6 antibody A01648-1 will work for ELISA with testis.
Boster Scientific Support
Answered: 2020-03-09
Question
Will A01648-1 anti-PLA2G6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
As indicated on the product datasheet, A01648-1 anti-PLA2G6 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-14
Question
We are currently using anti-PLA2G6 antibody A01648-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-PLA2G6 antibody (A01648-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
I was wanting to use your anti-PLA2G6 antibody for ELISA for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
G. Miller
Verified customer
Asked: 2019-06-28
Answer
You can see on the product datasheet, A01648-1 anti-PLA2G6 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-28
Question
I have a question about product A01648-1, anti-PLA2G6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01648-1 anti-PLA2G6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-PLA2G6 antibody (A01648-1)?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
We do provide the blocking peptide for product anti-PLA2G6 antibody (A01648-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-27
Question
Do you have a BSA free version of anti-PLA2G6 antibody A01648-1 available?
A. Johnson
Verified customer
Asked: 2016-11-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PLA2G6 antibody A01648-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-11-10
