Product Info Summary
SKU: | PA2278 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Plakophilin 2/PKP2 Antibody
View all Plakophilin 2 Antibodies
SKU/Catalog Number
PA2278
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Plakophilin 2/PKP2 Antibody catalog # PA2278. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plakophilin 2/PKP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2278)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Plakophilin 2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2278 is reactive to PKP2 in Human, Mouse, Rat
Applications
PA2278 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
97 kDa
Calculated molecular weight
97.415kDa
Background of Plakophilin 2
Plakophilin-2 is a protein that in humans is encoded by the PKP2 gene. This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. It is mapped to 12p11.21. PKP2 is a constituent of the desmosomal plaque in simple epithelia, some stratified epithelia, and some nonepithelial cells. PKP2 is also enriched in the karyoplasm of cells of various types, including those lacking desmosomes. Plakophilin proteins participate in linking cadherins to intermediate filaments in the cytoskeleton and this gene product may regulate the signaling activity of beta-catenin. PKP2 has been shown to interact with Desmoplakin, Plakoglobin and Desmoglein 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-Plakophilin 2 antibody, PA2278, All Western blotting
All lanes: Anti-PKP2(PA2278) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Intestine Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: U87 Whole Cell Lysate at 40ug
Predicted bind size: 97KD
Observed bind size: 97KD
Protein Target Info & Infographic
Gene/Protein Information For PKP2 (Source: Uniprot.org, NCBI)
Gene Name
PKP2
Full Name
Plakophilin-2
Weight
97.415kDa
Superfamily
beta-catenin family
Alternative Names
ARVD9; MGC177501; plakophilin 2; plakophilin-2 PKP2 ARVD9 plakophilin 2 plakophilin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PKP2, check out the PKP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PKP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Plakophilin 2/PKP2 Antibody (PA2278)
Hello CJ!
No publications found for PA2278
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Plakophilin 2/PKP2 Antibody
Question
We are currently using anti-Plakophilin 2/PKP2 antibody PA2278 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-Plakophilin 2/PKP2 antibody (PA2278) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17