Anti-Plasminogen PLG Antibody Peroxidase Conjugated

Product Name

Anti-Plasminogen PLG Antibody Peroxidase Conjugated

View all PLG Antibodies

SKU/Catalog Number

H00702

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Plasminogen PLG Antibody Peroxidase Conjugated (Catalog # H00702). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Plasminogen PLG Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H00702)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Clonality

Polyclonal

Isotype

IgG

Immunogen

Plasminogen [Human Plasma]

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

H00702 is reactive to PLG in Human

Applications

H00702 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

90.569kDa

Background of PLG

Plasmin is released as a zymogen called plasminogen (PLG) from the liver into the systemic circulation. Two major glycoforms of plasminogen are present in humans. Type II plasminogen is preferentially recruited to the cell surface over the type I glycoform. Conversely, type I plasminogen appears more readily recruited to blood clots. In circulation, plasminogen adopts a closed, activation resistant conformation. Upon binding to clots, or to the cell surface, plasminogen adopts an open form that can be converted into active plasmin by a variety of enzymes, including tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Fibrin is a cofactor for plasminogen activation by tissue plasminogen activator. Urokinase plasminogen activator receptor (uPAR) is a cofactor for plasminogen activation by urokinase plasminogen activator. The conversion of plasminogen to plasmin involves the cleavage of the peptide bond between Arg-561 and Val-562. Deficiency in plasmin may lead to thrombosis, as clots are not degraded adequately. Plasminogen deficiency in mice leads to defective liver repair, defective wound healing, reproductive abnormalities. In humans, a rare disorder called plasminogen deficiency type I is caused by mutations of the PLG gene and is often manifested by ligneous conjunctivitis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: 1:250 - 1:1,000
WB: 1:500 - 1:2,500

Validation Images & Assay Conditions

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Plasminogen under reducing (R) and non-reducing (NR) conditions were detected using goat anti-Plasminogen polyclonal antibody (Catalog # H00702) at 1:3000. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE.

Gene/Protein Information For PLG (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp779M0222; EC 3.4.21; EC 3.4.21.7; plasmin; Plasminogen; Plg PLG plasminogen plasminogen|plasmin

For more info on PLG, check out the PLG Infographic

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In this infographic, you will see the following information for PLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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