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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA)|
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Human Angiostatin Kringle 1-3/PLG ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Angiostatin Kringle 1-3/PLG ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLG in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Angiostatin Kringle 1-3/PLG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0905)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: V98-S355 (K1-3)
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0905 is reactive to PLG in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
EK0905 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PLG
Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED₅₀ =70 nM) relative to kringles 1-4 (ED₅₀ = 135 nM).
|EK0905-CAP||Anti-Human PLG Pre-coated 96-well strip microplate||1|
|EK0905-ST||Human PLG Standard||2 vials, 100 ng/tube|
|EK0905-DA||Human PLG Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Angiostatin K1-3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PLG, Dilution ratio of 1:1000, concentration in serum is around 1000ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Angiostatin Kringle 1-3/PLG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of PLG in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PLG (Source: Uniprot.Org, NCBI)
peptidase S1 family
DKFZp779M0222; EC 3.4.21; EC 220.127.116.11; plasmin; Plasminogen; Plg PLG plasminogen plasminogen|plasmin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PLG, check out the PLG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0905 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dysfunctional fibrinolysis and cerebral venous thrombosis
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