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- Table of Contents
PLLP antibody (A10793) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HepG2 cells using anti-Plasmolipin/PLLP antibody (A10793).
Overlay histogram showing HepG2 cells stained with A10793 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasmolipin/PLLP Antibody (A10793, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing HepG2 cells stained with A10793 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasmolipin/PLLP Antibody (A10793, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-Plasmolipin/PLLP Antibody Picoband®
PLLP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
PLLP antibody was positively detected by WB in:
human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PLLP antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-Plasmolipin/PLLP Antibody Picoband®
View all PLLP Antibodies
View all PLLP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Plasmolipin/PLLP recombinant protein (Position: M1-A142).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
26 kDa
Calculated Molecular Weight
20.0 kDa
Background of PLLP
PLLP (Plasmolipin, also known as PMLP; TM4SF11), located on 16q13, is a Protein Coding gene. The gene produces a 19987 Da protein composed of 182 amino acids. Plasmolipin (PLLP) is a novel protein identified in human corneal epithelium and has been demonstrated to have a key role in epithelial cell differentiation in other tissues. Plasmolipin is a hydrophobic plasma membrane proteolipid present in both the kidney and brain. The mouse plasmolipin gene has the common organization of the 4-transmembrane (4TM) gene group, with 4 exons and a large first intron.
Cite This Product
Anti-Plasmolipin/PLLP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10793)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
94%
High Homology
Mouse
89%
Moderate Homology
Rat
87%
Moderate Homology
Rabbit
87%
Moderate Homology
Cow
86%
Moderate Homology
Sheep
86%
Moderate Homology
Zebrafish
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PLLP antibody
Flow Cytometry (FC) protocol for PLLP antibody
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Product Promise
We promise Anti-Plasmolipin/PLLP Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A10793
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026