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Product Info Summary
| SKU: | A10904 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PLD6 Antibody (Center)
SKU/Catalog Number
A10904
Size
80 µl
Description
Boster Bio Anti-PLD6 Antibody (Center) (Catalog # A10904). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PLD6 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10904)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PLD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from of human PLD6.
Reactive Species
A10904 is reactive to PLD6 in Human, Mouse
Calculated molecular weight
28.3 kDa
Background of PLD6
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10904 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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PLD6 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane). This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).
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Western blot analysis of lysates from human ovary and mouse testis tissue lysates (from left to right), using PLD6 Antibody (Center) (Cat. #A10904). A10904 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 28 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 28 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-PLD6 Antibody (Center) (A10904)
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