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Product Info Summary
| SKU: | A11037-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PLEKHB1 Antibody Picoband®
SKU/Catalog Number
A11037-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PLEKHB1 Antibody Picoband® catalog # A11037-1. Tested in WB, ELISA applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PLEKHB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11037-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human PLEKHB1 recombinant protein (Position: H82-S214). Human PLEKHB1 shares 93.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PLEKHB1, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A11037-1 is reactive to PLEKHB1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.2 kDa
Background of PLEKHB1
PLEKHB1, also named as Evectin-1, EVT1, KPL1, PHR1 and PHRET1, isrequired for properlocalization of retinogeniculate projections but not for eye-specific segregation. PLEKHB1 has 4 isoforms with MW from 21-27kd. It has Dimerform withMW42-54 kDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11037-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human HepG2 whole cell, human U251 whole cell, monkey RF/6A whole cell, rat eye tissue, mouse eye tissue
Validation Images & Assay Conditions
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Western blot analysis of PLEKHB1 using anti-PLEKHB1 antibody (A11037-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: monkey RF/6A whole cell lysates,
Lane 5: rat eye tissue lysates,
Lane 6: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHB1 antigen affinity purified polyclonal antibody (Catalog # A11037-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHB1 at approximately 27 kDa. The expected band size for PLEKHB1 is at 27 kDa.
Specific Publications For Anti-PLEKHB1 Antibody Picoband® (A11037-1)
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