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Product Info Summary
| SKU: | A11280 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PLEKHM2 Antibody
SKU/Catalog Number
A11280
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PLEKHM2 Antibody (Catalog # A11280). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PLEKHM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PLEKHM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11280)
Host
Rabbit
Contents
PLEKHM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PLEKHM2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM2. The immunogen is located within the last 50 amino acids of PLEKHM2.
Reactive Species
A11280 is reactive to PLEKHM2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
112.8 kDa
Background of PLEKHM2
PLEKHM2, also known as SKIP, is a member of the M family of Pleckstrin homology domain-containing proteins. While little is known of PLEKHM2, a recent study examining differential gene expression in human hematopoietic stem cells has shown it to be specifically expressed in stem cells, suggesting that PLEKHM2 may play a role in erythroid commitment and development. Other studies have shown that PLEKHM2 is required for interaction with the Salmonella virulence factor SifA for Salmonella pathogenesis, suggesting that PLEKHM2 has cellular roles other than in the developing embryo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11280 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PLEKHM2 antibody can be used for detection of PLEKHM2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PLEKHM2 in rat brain tissue lysate with PLEKHM2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of PLEKHM2 in human liver tissue with FAM59A antibody at 5 μg/mL.
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Immunofluorescence of PLEKHM2 in Human Brain cells with PLEKHM2 antibody at 20 μg/mL.
Specific Publications For Anti-PLEKHM2 Antibody (A11280)
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