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Product Info Summary
| SKU: | M05322 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PLOD1 Antibody (C-term)
SKU/Catalog Number
M05322
Size
50 µl
Description
Boster Bio Anti-PLOD1 Antibody (C-term) (Catalog # M05322). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PLOD1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05322)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2114CT109.4.71.73
Isotype
IgG2b,κ
Immunogen
This PLOD1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 61-94 amino acids from the C-terminal region of human PLOD1.
Reactive Species
M05322 is reactive to PLOD1 in Human
Calculated molecular weight
83.6 kDa
Background of PLOD1
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05322 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-PLOD1 Antibody (C-term) at 1:4000 dilution
Lane 1: U-87MG whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: HeLa whole cell lysate
Lane 4: A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 84 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-PLOD1 Antibody (C-term) (M05322)
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