Anti-PMCA1 Rabbit Monoclonal Antibody

Product Name

Anti-PMCA1 Rabbit Monoclonal Antibody

View all PMCA1 Antibodies

SKU/Catalog Number

M02669

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-PMCA1 Rabbit Monoclonal Antibody catalog # M02669. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PMCA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02669)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

22A09

Isotype

IgG

Immunogen

A synthesized peptide derived from human PMCA1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02669 is reactive to ATP2B1 in Human, Mouse, Rat

Applications

M02669 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

130-250 kDa

Calculated molecular weight

134.685kDa

Background of PMCA1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PMCA1 using anti-PMCA1 antibody (M02669).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMCA1 antigen affinity purified monoclonal antibody (Catalog # M02669) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMCA1 at approximately 250 kDa. The expected band size for PMCA1 is at 135 kDa.

Gene/Protein Information For ATP2B1 (Source: Uniprot.org, NCBI)

Alternative Names

ATPase, Ca++ transporting, plasma membrane 1; EC 3.6.3; EC 3.6.3.8; Plasma membrane calcium ATPase isoform 1; plasma membrane calcium ATPase; Plasma membrane calcium pump isoform 1; plasma membrane calcium pump; plasma membrane calcium-ATPase; PMCA1kb; PMCA1plasma membrane calcium-transporting ATPase 1 ATP2B1 PMCA1, PMCA1kb ATPase plasma membrane Ca2+ transporting 1 plasma membrane calcium-transporting ATPase 1|ATPase, Ca++ transporting, plasma membrane 1|plasma membrane calcium pump

For more info on ATP2B1, check out the ATP2B1 Infographic

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In this infographic, you will see the following information for ATP2B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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