Product Info Summary
SKU: | A11017-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PNLIPRP2 Antibody Picoband™
View all Pancreatic Lipase Related Protein 2 Antibodies
SKU/Catalog Number
A11017-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PNLIPRP2 Antibody Picoband™ catalog # A11017-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PNLIPRP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11017-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PNLIPRP2 recombinant protein (Position: D30-C469).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11017-1 is reactive to PNLIPRP2 in Human, Mouse, Rat
Applications
A11017-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.961kDa
Background of Pancreatic Lipase Related Protein 2
This gene encodes a lipase that hydrolyzes galactolipids, the main components of plant membrane lipids. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the non-coding allele.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg /1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PNLIPRP2 using anti-PNLIPRP2 antibody (A11017-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNLIPRP2 antigen affinity purified polyclonal antibody (Catalog # A11017-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNLIPRP2 at approximately 52 kDa. The expected band size for PNLIPRP2 is at 52 kDa.
Click image to see more details
Figure 2. IF analysis of PNLIPRP2 using anti-PNLIPRP2 antibody (A11017-1).
PNLIPRP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PNLIPRP2 Antibody (A11017-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of Hela cells using anti-PNLIPRP2 antibody (A11017-1).
Overlay histogram showing Hela cells stained with A11017-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNLIPRP2 Antibody (A11017-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PNLIPRP2 (Source: Uniprot.org, NCBI)
Gene Name
PNLIPRP2
Full Name
Pancreatic lipase-related protein 2
Weight
51.961kDa
Superfamily
AB hydrolase superfamily
Alternative Names
EC 3.1.1; EC 3.1.1.3; galactolipase; pancreatic lipase-related protein 2; PL-RP2; PLRP2EC 3.1.1.26 PNLIPRP2 PLRP2 pancreatic lipase related protein 2 (gene/pseudogene) pancreatic lipase-related protein 2|PL-RP2|cytotoxic T lymphocyte lipase|galactolipase|triacylglycerol lipase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PNLIPRP2, check out the PNLIPRP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PNLIPRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PNLIPRP2 Antibody Picoband™ (A11017-1)
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