Click image to see more details
Product Info Summary
| SKU: | A08812-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-POLM Antibody Picoband®
SKU/Catalog Number
A08812-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-POLM Antibody Picoband® catalog # A08812-2. Tested in WB, IP, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-POLM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08812-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human POLM recombinant protein (Position: E36-H481). Human POLM shares 76.5% amino acid (aa) sequence identity with mouse POLM.
Reactive Species
A08812-2 is reactive to POLM in Human
Observed Molecular Weight
55 kDa
Calculated molecular weight
54.8 kDa
Background of POLM
Predicted to enable DNA-directed DNA polymerase activity. Predicted to be involved in double-strand break repair via nonhomologous end joining. Predicted to act upstream of or within B cell differentiation and somatic hypermutation of immunoglobulin genes. Predicted to be located in nucleoplasm. Predicted to be active in nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08812-2 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of POLM using anti-POLM antibody (A08812-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLM antigen affinity purified polyclonal antibody (A08812-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLM at approximately 55 kDa. The expected band size for POLM is at 55 kDa.
Click image to see more details
Immunoprecipitating POLM in 293T whole cell lysate.
Western blot analysis of POLM using anti-POLM antibody (A08812-2).
Lane 1: 293T whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-POLM antibody in 293T whole cell lysate,
Lane 3: anti-POLM antibody (2μg) + 293T whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLM antigen affinity purified polyclonal antibody (A08812-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POLM at approximately 55 kDa. The expected band size for POLM is at 55 kDa.
Specific Publications For Anti-POLM Antibody Picoband® (A08812-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-POLM Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-POLM Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
