Anti-POLR1B/Rpa135 Antibody

Product Name

Anti-POLR1B/Rpa135 Antibody

View all Polr1b Antibodies

SKU/Catalog Number

A11908

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-POLR1B/Rpa135 Antibody (Catalog # A11908). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-POLR1B/Rpa135 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11908)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human POLR1B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11908 is reactive to Polr1b in Human, Mouse, Rat

Applications

A11908 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

128.213kDa

Background of Polr1b

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.

Ota T., Nat. Genet. 36:40-45(2004).
Hillier L.W., Nature 434:724-731(2005).
Miller G., EMBO J. 20:1373-1382(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from Jurkat/NIH-3T3 cells, using POLR1B antibody A11908.

Gene/Protein Information For Polr1b (Source: Uniprot.org, NCBI)

Alternative Names

DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; EC 2.7.7.6; FLJ10816; FLJ21921; MGC131780; polymerase (RNA) I polypeptide B, 128kDa; RNA polymerase I subunit 2; RPA116; RPA135; RPA2; Rpo1-2 Polr1b|128kD, 128kDa, D630020H17Rik, R, RPA, RPA116, RPA135, RPA2, Rpo, Rpo1-2|polymerase (RNA) I polypeptide B|DNA-directed RNA polymerase I subunit RPA2|DNA-directed RNA polymerase I 135 kDa polypeptide|DNA-directed RNA polymerase I 135kDa polypeptide|RNA polymerase 1-2, 128 kDa subunit|RNA polymerase I subunit 2

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