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Product Info Summary
| SKU: | A11077 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-POLR1E Antibody
SKU/Catalog Number
A11077
Size
100ul
Form
Liquid
Description
Boster Bio Anti-POLR1E Antibody catalog # A11077. Tested in WB,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLR1E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11077)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human POLR1E(NP_071935.1).
Reactive Species
A11077 is reactive to POLR1E in Human, Mouse, Rat
Calculated molecular weight
47.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11077 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IP: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using POLR1E antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
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Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody . Western blot was performed from the immunoprecipitate using POLR1E antibody at a dilution of 1:1000.
Specific Publications For Anti-POLR1E Antibody (A11077)
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