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Product Info Summary
| SKU: | A09308-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-POLR2C Antibody (C-term)
SKU/Catalog Number
A09308-1
Size
80 µl
Description
Boster Bio Anti-POLR2C Antibody (C-term) (Catalog # A09308-1). Tested in IF, WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-POLR2C Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09308-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C.
Reactive Species
A09308-1 is reactive to POLR2C in Human, Mouse
Calculated molecular weight
31.4 kDa
Background of POLR2C
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09308-1 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:100
Validation Images & Assay Conditions
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Fluorescent image of Hela cells stained with POLR2C Antibody (C-term). A09308-1 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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POLR2C Antibody (C-term) western blot analysis in Hela,U251 cell line and mouse skeletal muscle tissue lysates (35ug/lane).This demonstrates the POLR2C antibody detected the POLR2C protein (arrow).
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Formalin-fixed and paraffin-embedded H.brain, H.cerebellum tissue reacted with POLR2C Antibody (C-term).
Specific Publications For Anti-POLR2C Antibody (C-term) (A09308-1)
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