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Product Info Summary
| SKU: | A01345 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Polycomb protein EED EED Antibody
SKU/Catalog Number
A01345
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Polycomb protein EED EED Antibody (Catalog # A01345). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EED antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Polycomb protein EED EED Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01345)
Host
Rabbit
Contents
EED Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
EED antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EED. The immunogen is located within amino acids 30 - 80 of EED.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Four alternatively spliced transcript variants have been observed.
Reactive Species
A01345 is reactive to EED in Human, Mouse, Rat
Applications
A01345 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.198kDa
Background of EED
The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin β7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EED antibody can be used for detection of EED by Western blot at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EED in human heart tissue lysate with EED antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of EED in human heart tissue with EED antibody at 20 μg/mL.
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Western blot analysis of EED expression in human testis tissue lysate with EED antibody at 2 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For EED (Source: Uniprot.org, NCBI)
Gene Name
EED
Full Name
Polycomb protein EED
Weight
50.198kDa
Superfamily
WD repeat ESC family
Alternative Names
EED; embryonic ectoderm development; HEED; polycomb protein EED; WAIT1 ; WAIT1; WAIT-1; WD protein associating with integrin cytoplasmic tails 1 EED COGIS, HEED, WAIT1 embryonic ectoderm development polycomb protein EED|WD protein associating with integrin cytoplasmic tails 1|embryonic ectoderm development protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EED, check out the EED Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EED: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Polycomb protein EED EED Antibody (A01345)
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No publications found for A01345
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Customer Reviews
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