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Product Info Summary
| SKU: | A04783 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-POU3F2 Antibody
SKU/Catalog Number
A04783
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-POU3F2 Antibody (Catalog # A04783). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
POU3F2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-POU3F2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04783)
Host
Rabbit
Contents
POU3F2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal POU3F2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human POU3F2. The immunogen is located within the last 50 amino acids of POU3F2.
Cross-reactivity
At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family.
Reactive Species
A04783 is reactive to POU3F2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.9 kDa
Background of POU3F2
POU3F2, also commonly known as brain-2, is a member of a family of POU domain genes expressed in mouse brain and is thought to be involved in the development of the neocortex and establishment of neural cell lineage. Recent studies suggest that POU3F2 may be involved with the development of neurodegenerative diseases as well as tumor development and proliferation. Along with the neural-lineage-specific transcription factors ASCL1 and MYT1L, POU3F2 can convert fibroblasts to functional neurons in vitro, a form of artificial stem cells termed induced neuronal (iN) cells, suggesting that these cells may be useful in the treatment of neurodegenerative diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04783 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
POU3F2 antibody can be used for detection of POU3F2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of POU3F2 in 3T3 cell lysate with POU3F2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of POU3F2 in human brain tissue with POU3F2 antibody at 20 μg/mL.
Specific Publications For Anti-POU3F2 Antibody (A04783)
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