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- Table of Contents
PPP2CA antibody (PB9347) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of K562 cells using anti-PPP2CA antibody (PB9347).
Overlay histogram showing K562 cells stained with PB9347 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2CA Antibody (PB9347, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing K562 cells stained with PB9347 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2CA Antibody (PB9347, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-PP2A-alpha/PPP2CA Antibody Picoband®
PPP2CA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
PPP2CA antibody was positively detected by WB in:
human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell, human Hela whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PPP2CA antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PPP2CA antibody was positively detected by ICC/IF in:
U2OS cell
PPP2CA antibody was positively detected by FC in:
K562 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Fruit fly
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PP2A-alpha/PPP2CA Antibody Picoband®
View all PPP2CA Antibodies
View all PPP2CA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309). Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.
Cross-Reactivity
PP2A-alpha shares 100% cross-reactivity with PPP2CB.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa, 36 kDa
Calculated Molecular Weight
35.6 kDa
Background of PPP2CA
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Cite This Product
Anti-PP2A-alpha/PPP2CA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9347)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Dog
99%
High Homology
Zebrafish
97%
High Homology
Drosophila
94%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PPP2CA antibody
Immunohistochemistry (IHC) protocol for PPP2CA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PPP2CA antibody
Flow Cytometry (FC) protocol for PPP2CA antibody
Publications
Application:
Species:
Essential roles of Akt/Snail pathway in microcystin-LR-induced tight junction toxicity in Sertoli cell
Published: 2018-01-04
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 3.98
Species: Mouse
PMID: 29307602
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PB9347
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 20, 2026