Anti-PP2C kappa PPM1K Antibody

Boster Bio Anti-PP2C kappa PPM1K Antibody catalog # A04319. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A04319
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-PP2C kappa PPM1K Antibody

See all PPM1K products

SKU/Catalog Number

A04319

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PP2C kappa PPM1K Antibody catalog # A04319. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PP2C kappa PPM1K Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04319)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Reactive Species

A04319 is reactive to PPM1K in Human

Applications

A04319 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PPM1K (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8N3J5

Gene ID

152926

Gene Name

PPM1K

Full Name

Protein phosphatase 1K, mitochondrial

Weight

40.997kDa

Superfamily

PP2C family

Alternative Names

DKFZp667B084; DKFZp761G058; mitochondrial; PP2C domain-containing protein phosphatase 1K; PP2Ckappa; PP2C-like mitochondrial protein; PP2CM; PP2C-type mitochondrial phosphoprotein phosphatase; protein phosphatase 2C kappa; protein phosphatase, Mg2+/Mn2+ dependent, 1K; PTMPEC 3.1.3.16 PPM1K BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07 protein phosphatase, Mg2+/Mn2+ dependent 1K protein phosphatase 1K, mitochondrial|PP2C domain-containing protein phosphatase 1K|PP2C-kappa|PP2C-type mitochondrial phosphoprotein phosphatase|branched-chain I+/--ketoacid dehydrogenase phosphatase|branched-chain alpha-ketoacid dehydrogenase phosphatase|protein phosphatase 2C kappa

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PPM1K, check out the PPM1K Infographic

PPM1K infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PPM1K: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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11 Customer Q&As for Anti-PP2C kappa PPM1K Antibody

Question

My question regarding product A04319, anti-PP2C kappa antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04319 anti-PP2C kappa antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-19

Question

Please see the WB image, lot number and protocol we used for amygdala lymph node using anti-PP2C kappa antibody A04319. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-24

Question

I see that the anti-PP2C kappa antibody A04319 works with WB, what is the protocol used to produce the result images on the product page?

K. Wu

Verified customer

Asked: 2020-01-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-16

Question

Would anti-PP2C kappa antibody A04319 work for WB with amygdala lymph node?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

According to the expression profile of amygdala lymph node, PPM1K is highly expressed in amygdala lymph node. So, it is likely that anti-PP2C kappa antibody A04319 will work for WB with amygdala lymph node.

Boster Scientific Support

Answered: 2019-12-30

Question

Would A04319 anti-PP2C kappa antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

As indicated on the product datasheet, A04319 anti-PP2C kappa antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-29

Question

I was wanting to use your anti-PP2C kappa antibody for WB for human amygdala lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amygdala lymph node identification?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

As indicated on the product datasheet, A04319 anti-PP2C kappa antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala lymph node in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-11

Question

Will anti-PP2C kappa antibody A04319 work on monkey WB with cerebellum testis?

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

Our lab technicians have not validated anti-PP2C kappa antibody A04319 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-PP2C kappa antibody A04319 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cerebellum testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-15

Question

I am looking for to test anti-PP2C kappa antibody A04319 on human amygdala lymph node for research purposes, then I may be interested in using anti-PP2C kappa antibody A04319 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-28

Answer

The products we sell, including anti-PP2C kappa antibody A04319, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-28

Question

Do you have a BSA free version of anti-PP2C kappa antibody A04319 available?

D. Edwards

Verified customer

Asked: 2017-03-27

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PP2C kappa antibody A04319 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-03-27

Question

Is a blocking peptide available for product anti-PP2C kappa antibody (A04319)?

C. Zhang

Verified customer

Asked: 2017-02-03

Answer

We do provide the blocking peptide for product anti-PP2C kappa antibody (A04319). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-02-03

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala lymph node using anti-PP2C kappa antibody A04319. Let me know if you need anything else.

M. Krishna

Verified customer

Asked: 2013-09-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-09-11

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04319
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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