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Product Info Summary
| SKU: | A04319 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PP2C kappa PPM1K Antibody
SKU/Catalog Number
A04319
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PP2C kappa PPM1K Antibody catalog # A04319. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP2C kappa PPM1K Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04319)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human TLE3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04319 is reactive to PPM1K in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
40997 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04319 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Positive Control
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using PP2Ckappa Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PPM1K (Source: Uniprot.org, NCBI)
Gene Name
PPM1K
Full Name
Protein phosphatase 1K, mitochondrial
Weight
40997 MW
Superfamily
PP2C family
Alternative Names
DKFZp667B084; DKFZp761G058; mitochondrial; PP2C domain-containing protein phosphatase 1K; PP2Ckappa; PP2C-like mitochondrial protein; PP2CM; PP2C-type mitochondrial phosphoprotein phosphatase; protein phosphatase 2C kappa; protein phosphatase, Mg2+/Mn2+ dependent, 1K; PTMPEC 3.1.3.16 PPM1K BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07 protein phosphatase, Mg2+/Mn2+ dependent 1K protein phosphatase 1K, mitochondrial|PP2C domain-containing protein phosphatase 1K|PP2C-kappa|PP2C-type mitochondrial phosphoprotein phosphatase|branched-chain I+/--ketoacid dehydrogenase phosphatase|branched-chain alpha-ketoacid dehydrogenase phosphatase|protein phosphatase 2C kappa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPM1K, check out the PPM1K Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPM1K: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP2C kappa PPM1K Antibody (A04319)
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No publications found for A04319
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-PP2C kappa PPM1K Antibody
Question
My question regarding product A04319, anti-PP2C kappa antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04319 anti-PP2C kappa antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-19
Question
Please see the WB image, lot number and protocol we used for amygdala lymph node using anti-PP2C kappa antibody A04319. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-24
Question
I see that the anti-PP2C kappa antibody A04319 works with WB, what is the protocol used to produce the result images on the product page?
K. Wu
Verified customer
Asked: 2020-01-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-16
Question
Would anti-PP2C kappa antibody A04319 work for WB with amygdala lymph node?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
According to the expression profile of amygdala lymph node, PPM1K is highly expressed in amygdala lymph node. So, it is likely that anti-PP2C kappa antibody A04319 will work for WB with amygdala lymph node.
Boster Scientific Support
Answered: 2019-12-30
Question
Would A04319 anti-PP2C kappa antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
As indicated on the product datasheet, A04319 anti-PP2C kappa antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-29
Question
I was wanting to use your anti-PP2C kappa antibody for WB for human amygdala lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amygdala lymph node identification?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
As indicated on the product datasheet, A04319 anti-PP2C kappa antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
Will anti-PP2C kappa antibody A04319 work on monkey WB with cerebellum testis?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
Our lab technicians have not validated anti-PP2C kappa antibody A04319 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-PP2C kappa antibody A04319 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cerebellum testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-15
Question
I am looking for to test anti-PP2C kappa antibody A04319 on human amygdala lymph node for research purposes, then I may be interested in using anti-PP2C kappa antibody A04319 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
The products we sell, including anti-PP2C kappa antibody A04319, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-28
Question
Do you have a BSA free version of anti-PP2C kappa antibody A04319 available?
D. Edwards
Verified customer
Asked: 2017-03-27
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PP2C kappa antibody A04319 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-03-27
Question
Is a blocking peptide available for product anti-PP2C kappa antibody (A04319)?
C. Zhang
Verified customer
Asked: 2017-02-03
Answer
We do provide the blocking peptide for product anti-PP2C kappa antibody (A04319). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala lymph node using anti-PP2C kappa antibody A04319. Let me know if you need anything else.
M. Krishna
Verified customer
Asked: 2013-09-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-11
