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Product Info Summary
| SKU: | A13059 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PPAPDC1B PLPP5 Antibody
SKU/Catalog Number
A13059
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PPAPDC1B PLPP5 Antibody (Catalog # A13059). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
PPAPDC1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PPAPDC1B PLPP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13059)
Host
Rabbit
Contents
PPAPDC1B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PPAPDC1B antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human PPAPDC1B. The immunogen is located within amino acids 30 - 80 of PPAPDC1B.
Reactive Species
A13059 is reactive to PPAPDC1B in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.5 kDa
Background of PPAPDC1B
Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide (NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. Knockdown experiments indicated that this protein is involved with multiple cell signaling pathways, including the JAK-Stat3, MAP kinase, and PKC pathways. PPAPDC1B may also potentiate the estrogen receptor pathway by down-regulating DUSP22.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13059 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PPAPDC1B antibody can be used for detection of PPAPDC1B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PPAPDC1B in EL4 cell lysate with PPAPDC1B antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-PPAPDC1B PLPP5 Antibody (A13059)
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