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Product Info Summary
| SKU: | A15380 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-PPAPDC3 PLPP7 Antibody
SKU/Catalog Number
A15380
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PPAPDC3 PLPP7 Antibody (Catalog # A15380). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PPAPDC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PPAPDC3 PLPP7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15380)
Host
Rabbit
Contents
PPAPDC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PPAPDC3 antibody was raised against a 19 amino acid synthetic peptide from near the amino of terminus human PPAPDC3. The immunogen is located within the first 50 amino acids of PPAPDC3.
Reactive Species
A15380 is reactive to PPAPDC3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.4 kDa
Background of PPAPDC3
PPAPDC3, also known as nuclear envelope transmembrane protein 39 (NET39), was initially discovered in an in silico screen for secreted or membrane proteins. It is a member of the PAP2 superfamily of phosphatases and haloperoxidases. PPAPDC3 has recently been shown to act as a negative regulator of myoblast differentiation by diminishing the activity of the mammalian target of rapamycin TOR. PPAPDC3 is highly expressed in cardiac and skeletal muscle and becomes strongly upregulated during cultured myoblast differentiation tissues. Overexpression of PPAPDC3 in myoblasts repressed myogenesis while knockdown by RNA interference promoted differentiation indicating its part in the regulatory mechanism for myogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15380 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PPAPDC3 antibody can be used for detection of PPAPDC3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PPAPDC3 in mouse heart tissue lysate with PPAPDC3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of PPAPDC3 in mouse heart tissue with PPAPDC3 antibody at 20 μg/mL.
Specific Publications For Anti-PPAPDC3 PLPP7 Antibody (A15380)
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