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Product Info Summary
| SKU: | A00236 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PPARGC1A Antibody
View all PGC1 alpha Antibodies
SKU/Catalog Number
A00236
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PPARGC1A Antibody (Catalog # A00236). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PPARGC1A antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PPARGC1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00236)
Host
Rabbit
Contents
PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
PPARGC1A antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PPARGC1A. The immunogen is located within the last 50 amino acids of PPARGC1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody only detects the longest isoform.
Reactive Species
A00236 is reactive to PPARGC1A in Human, Mouse, Rat
Applications
A00236 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
91.027kDa
Background of PGC1 alpha
The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PPARGC1A antibody can be used for detection of PPARGC1A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PPARGC1A in mouse liver tissue lysate with PPARGC1A antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of PPARGC1A in human liver tissue with PPARGC1A antibody at 5 μg/mL.
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Immunofluorescence of PPARGC1A in human liver tissue with PPARGC1A antibody at 20 μg/mL.
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Western blot analysis of PPARGC1A expression in human liver tissue lysate with PPARGC1A antibody at (A) 1 and (B) 2 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PPARGC1A (Source: Uniprot.org, NCBI)
Gene Name
PPARGC1A
Full Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
Weight
91.027kDa
Alternative Names
LEM6; Ligand effect modulator 6; ligand effect modulator-6; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC1 alpha; PGC1A; PGC-1-alpha; PGC1APGC-1(alpha); PGC1peroxisome proliferative activated receptor, gamma, coactivator 1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPAR-gamma coactivator 1-alpha; PPARGC1A; PPARGC-1-alpha; PPARGC1PGC-1v PPARGC1A LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1 PPARG coactivator 1 alpha peroxisome proliferator-activated receptor gamma coactivator 1-alpha|L-PGC-1alpha|PGC-1-alpha|PPAR gamma coactivator variant form|PPARGC-1-alpha|PPARgamma coactivator 1alpha|ligand effect modulator-6|peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext|peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a|peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPARGC1A, check out the PPARGC1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPARGC1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPARGC1A Antibody (A00236)
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