Product Info Summary
SKU: | A01576-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PPM1D/WIP1 Picoband™ Antibody
SKU/Catalog Number
A01576-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PPM1D/WIP1 Picoband™ Antibody catalog # A01576-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPM1D/WIP1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01576-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PPM1D/WIP1 recombinant protein (Position: R18-C605).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01576-1 is reactive to PPM1D in Human, Mouse, Rat
Applications
A01576-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
66.675kDa
Background of PPM1D
Protein phosphatase 1D is an enzyme that in humans is encoded by the PPM1D gene. It is mapped to 17q23.2. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PPM1D using anti-PPM1D antibody (A01576-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MDA-MB-453 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1D antigen affinity purified polyclonal antibody (Catalog # A01576-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1D at approximately 75KD. The expected band size for PPM1D is at 67KD.
Click image to see more details
Figure 2. IF analysis of PPM1D using anti-PPM1D antibody (A01576-1).
PPM1D was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PPM1D Antibody (A01576-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of A431 cells using anti-PPM1D antibody (A01576-1).
Overlay histogram showing A431 cells stained with A01576-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1D Antibody (A01576-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PPM1D (Source: Uniprot.org, NCBI)
Gene Name
PPM1D
Full Name
Protein phosphatase 1D
Weight
66.675kDa
Superfamily
PP2C family
Alternative Names
EC 3.1.3.16; p53-induced protein phosphatase 1; PP2C-delta; PPM1D; protein phosphatase 1D magnesium-dependent, delta isoform; protein phosphatase 1D; protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium-dependent 1 delta; protein phosphatase, Mg2+/Mn2+ dependent, 1D; wild-type p53-induced phosphatase 1; Wip1; WIP1protein phosphatase Wip1 PPM1D IDDGIP, JDVS, PP2C-DELTA, WIP1 protein phosphatase, Mg2+/Mn2+ dependent 1D protein phosphatase 1D|protein phosphatase Wip1|wild-type p53-induced phosphatase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPM1D, check out the PPM1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPM1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPM1D/WIP1 Picoband™ Antibody (A01576-1)
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