Anti-PPM1F Antibody Picoband™

Product Name

Anti-PPM1F Antibody Picoband™

View all Protein phosphatase 1F Antibodies

SKU/Catalog Number

A07776-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPM1F Antibody Picoband™ catalog # A07776-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PPM1F Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07776-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PPM1F recombinant protein (Position: E17-S454).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07776-1 is reactive to PPM1F in Human

Applications

A07776-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

49.831kDa

Background of Protein phosphatase 1F

Protein phosphatase 1F is an enzyme that in humans is encoded by the PPM1F gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PPM1F using anti-PPM1F antibody (A07776-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1F antigen affinity purified polyclonal antibody (Catalog # A07776-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1F at approximately 54 kDa. The expected band size for PPM1F is at 50 kDa.

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Figure 2. IF analysis of PPM1F using anti-PPM1F antibody (A07776-1) and anti-Beta Tubulin antibody (M01857-3).
PPM1F was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPM1F Antibody (A07776-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PPM1F (Source: Uniprot.org, NCBI)

Alternative Names

Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaMKPaseCaM-kinase phosphatase; EC 3.1.3.16; FEM-2; hFEM-2; KIAA0015POPX2CAMKP; Partner of PIX 2; PP2C phosphatase; Protein fem-2 homolog; protein phosphatase 1F (PP2C domain containing); protein phosphatase 1F; protein phosphatase, Mg2+/Mn2+ dependent, 1F PPM1F CAMKP, CaMKPase, FEM-2, POPX2, hFEM-2 protein phosphatase, Mg2+/Mn2+ dependent 1F protein phosphatase 1F|Ca(2+)/calmodulin-dependent protein kinase phosphatase|CaM-kinase phosphatase|PP2C phosphatase|partner of PIX 2|protein fem-2 homolog|protein phosphatase 1F (PP2C domain containing)

For more info on PPM1F, check out the PPM1F Infographic

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In this infographic, you will see the following information for PPM1F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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