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Product Info Summary
| SKU: | M03058 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-PPP1R13L Antibody (N-Term)
SKU/Catalog Number
M03058
Size
80 µl
Description
Boster Bio Anti-PPP1R13L Antibody (N-Term) (Catalog # M03058). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PPP1R13L Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03058)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Clone Number
153CT43.1.1
Isotype
Rabbit IgG
Immunogen
This PPP1R13L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from human PPP1R13L.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03058 is reactive to PPP1R13L in Human
Applications
M03058 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
89.091kDa
Background of iASPP
Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling PPP1R13L with M03058 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-PPP1R13L Antibody (N-Term) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: NCI-H1299 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 89 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R13L (Source: Uniprot.org, NCBI)
Gene Name
PPP1R13L
Full Name
RelA-associated inhibitor
Weight
89.091kDa
Superfamily
iASPP family
Alternative Names
IASPPinhibitor of apoptosis stimulating protein of p53; Inhibitor of ASPP protein; NFkB-interacting protein 1; NKIP1; PPP1R13BL; PPP1R13B-like protein; Protein iASPP; protein phosphatase 1, regulatory (inhibitor) subunit 13 like; protein phosphatase 1, regulatory subunit 13 like; RAINFkB interacting protein 1; relA-associated inhibitor PPP1R13L IASPP, NKIP1, RAI, RAI4 protein phosphatase 1 regulatory subunit 13 like relA-associated inhibitor|NFkB interacting protein 1|PPP1R13B-like protein|inhibitor of ASPP protein|inhibitor of apoptosis stimulating protein of p53|protein iASPP|protein phosphatase 1, regulatory (inhibitor) subunit 13 like|retinoic acid induced 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R13L, check out the PPP1R13L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R13L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP1R13L Antibody (N-Term) (M03058)
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No publications found for M03058
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Customer Reviews
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