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Product Info Summary
| SKU: | A04159 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-PPP2R2A Antibody (N-term)
SKU/Catalog Number
A04159
Size
80 µl
Description
Boster Bio Anti-PPP2R2A Antibody (N-term) (Catalog # A04159). Tested in IHC-P, IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PPP2R2A Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04159)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A.
Reactive Species
A04159 is reactive to PPP2R2A in Human
Calculated molecular weight
51.7 kDa
Background of PPP2R2A
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04159 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Fluorescent image of Hela cells stained with PPP2R2A Antibody (N-term). A04159 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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PPP2R2A Antibody (N-term) (Cat. #A04159) western blot analysis in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the PPP2R2A antibody detected the PPP2R2A protein (arrow).
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Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term) (Cat#A04159). A04159 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term) (Cat#A04159). A04159 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Specific Publications For Anti-PPP2R2A Antibody (N-term) (A04159)
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