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Product Info Summary
| SKU: | A11897-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PPP2R3C/G5Pr Antibody
SKU/Catalog Number
A11897-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PPP2R3C/G5Pr Antibody catalog # A11897-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP2R3C/G5Pr Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11897-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PPP2R3C. AA range:165-214
Reactive Species
A11897-1 is reactive to PPP2R3C in Human, Mouse, Rat
Calculated molecular weight
53.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11897-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western blot analysis of PPP2R3C Antibody. The lane on the right is blocked with the PPP2R3C peptide.
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Western blot analysis of the lysates from HepG2 cells using PPP2R3C antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-PPP2R3C/G5Pr Antibody (A11897-1)
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Customer Q&As
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7 Customer Q&As for Anti-PPP2R3C/G5Pr Antibody
Question
We are currently using anti-PPP2R3C/G5Pr antibody A11897-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-PPP2R3C/G5Pr antibody (A11897-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
Question
Would anti-PPP2R3C/G5Pr antibody A11897-1 work for WB with cerebellum gastric carcinoma?
P. Jackson
Verified customer
Asked: 2019-11-28
Answer
According to the expression profile of cerebellum gastric carcinoma, PPP2R3C is highly expressed in cerebellum gastric carcinoma. So, it is likely that anti-PPP2R3C/G5Pr antibody A11897-1 will work for WB with cerebellum gastric carcinoma.
Boster Scientific Support
Answered: 2019-11-28
Question
See below the WB image, lot number and protocol we used for cerebellum gastric carcinoma using anti-PPP2R3C/G5Pr antibody A11897-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-30
Question
Will A11897-1 anti-PPP2R3C/G5Pr antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
It shows on the product datasheet, A11897-1 anti-PPP2R3C/G5Pr antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum gastric carcinoma using anti-PPP2R3C/G5Pr antibody A11897-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-29
Question
I was wanting to use your anti-PPP2R3C/G5Pr antibody for WB for mouse cerebellum gastric carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cerebellum gastric carcinoma identification?
F. Thomas
Verified customer
Asked: 2017-09-12
Answer
You can see on the product datasheet, A11897-1 anti-PPP2R3C/G5Pr antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cerebellum gastric carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-12
Question
Is this A11897-1 anti-PPP2R3C/G5Pr antibody reactive to the isotypes of PPP2R3C?
N. Zhao
Verified customer
Asked: 2015-04-08
Answer
The immunogen of A11897-1 anti-PPP2R3C/G5Pr antibody is Synthesized peptide derived from the Internal region of human PPP2R3C. at AA rangle: 140-220. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-04-08
