Anti-PPP2R5D Antibody Picoband™

Product Name

Anti-PPP2R5D Antibody Picoband™

View all PPP2R5D Antibodies

SKU/Catalog Number

A06450-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPP2R5D Antibody Picoband™ catalog # A06450-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PPP2R5D Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06450-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PPP2R5D recombinant protein (Position: R91-L602).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06450-1 is reactive to PPP2R5D in Human, Mouse, Rat

Applications

A06450-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

74 kDa

Calculated molecular weight

69.992kDa

Background of PPP2R5D

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform is an enzyme that in humans is encoded by the PPP2R5D gene. The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PPP2R5D using anti-PPP2R5D antibody (A06450-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5D antigen affinity purified polyclonal antibody (Catalog # A06450-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R5D at approximately 74 kDa. The expected band size for PPP2R5D is at 70 kDa.

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Figure 2. Flow Cytometry analysis of SiHa cells using anti-PPP2R5D antibody (A06450-1).
Overlay histogram showing SiHa cells stained with A06450-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R5D Antibody (A06450-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PPP2R5D (Source: Uniprot.org, NCBI)

Alternative Names

MGC2134; MGC8949; PP2A B subunit isoform B56-delta; PP2A B subunit isoform B'-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta; PP2A, B subunit, B' delta isoform; PP2A, B subunit, B56 delta isoform; PP2A, B subunit, PR61 delta isoform; PP2A, B subunit, R5 delta isoform; protein phosphatase 2, regulatory subunit B', delta; regulatory subunit B (B56), delta isoform; regulatory subunit B', delta isoform; Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, deltaisoform; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform PPP2R5D B56D, B56delta, MRD35 protein phosphatase 2 regulatory subunit Bdelta serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform

For more info on PPP2R5D, check out the PPP2R5D Infographic

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In this infographic, you will see the following information for PPP2R5D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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