Anti-PPP3CC Antibody Picoband™

Product Name

Anti-PPP3CC Antibody Picoband™

View all PPP3CC Antibodies

SKU/Catalog Number

A07461-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPP3CC Antibody Picoband™ catalog # A07461-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PPP3CC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07461-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human PPP3CC recombinant protein (Position: Q24-S512).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07461-1 is reactive to PPP3CC in Human, Mouse, Rat

Applications

A07461-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

58.129kDa

Background of PPP3CC

Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (PP2BC) is an enzyme that in humans is encoded by the PPP3CC gene. Calcineurin is a calcium-dependent, calmodulin-stimulated protein phosphatase involved in the downstream regulation of dopaminergic signal transduction. Calcineurin is composed of a regulatory subunit and a catalytic subunit. The protein encoded by this gene represents one of the regulatory subunits that has been found for calcineurin. Three transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg /1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PPP3CC using anti-PPP3CC antibody (A07461-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CC antigen affinity purified polyclonal antibody (Catalog # A07461-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3CC at approximately 65 kDa. The expected band size for PPP3CC is at 58 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-PPP3CC antibody (A07461-1).
Overlay histogram showing HepG2 cells stained with A07461-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP3CC Antibody (A07461-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PPP3CC (Source: Uniprot.org, NCBI)

Alternative Names

Calcineurin, testis-specific catalytic subunit; Calmodulin-dependent calcineurin A subunit gamma isoform; CALNA3CAM-PRP catalytic subunit; CNA3; EC 3.1.3.16; PP2Bgamma; protein phosphatase 2B, catalytic subunit, gamma isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform(calcineurin A gamma); protein phosphatase 3, catalytic subunit, gamma isozyme; serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform PPP3CC CALNA3, CNA3, PP2Bgamma protein phosphatase 3 catalytic subunit gamma serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform|CAM-PRP catalytic subunit|calcineurin, testis-specific catalytic subunit|protein phosphatase 3, catalytic subunit, gamma isozyme

For more info on PPP3CC, check out the PPP3CC Infographic

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In this infographic, you will see the following information for PPP3CC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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