Anti-PRC1 Antibody

Product Name

Anti-PRC1 Antibody

View all PRC1 Antibodies

SKU/Catalog Number

A00160

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PRC1 Antibody (Catalog # A00160). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PRC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PRC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00160)

Host

Rabbit

Contents

PRC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1. The immunogen is located within amino acids 350 - 400 of PRC1.

Cross-reactivity

At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Reactive Species

A00160 is reactive to PRC1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

71.6 kDa

Background of PRC1

PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00160 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PRC1

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