Click image to see more details
Product Info Summary
| SKU: | M01845 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01845
BM4871 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal Antibody catalog # M01845. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01845)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IIG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PRDX1
Reactive Species
M01845 is reactive to PRDX1 in Human, Mouse
Observed Molecular Weight
24 kDa
Calculated molecular weight
22.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01845 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Validation Images & Assay Conditions
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Western blot analysis of PRDX1/Peroxiredoxin 1 using anti-PRDX1/Peroxiredoxin 1 antibody (M01845).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX1/Peroxiredoxin 1 antigen affinity purified monoclonal antibody (Catalog # M01845) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX1/Peroxiredoxin 1 at approximately 24 kDa. The expected band size for PRDX1/Peroxiredoxin 1 is at 22,24 kDa.
Specific Publications For Anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal Antibody (M01845)
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Customer Q&As
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6 Customer Q&As for Anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody (M01845)?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
We do provide the blocking peptide for product anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody (M01845). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-20
Question
Here is the WB image, lot number and protocol we used for brain using anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-10
Question
Does anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
According to the expression profile of brain, PRDX1 is highly expressed in brain. So, it is likely that anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845 will work for WB with brain.
Boster Scientific Support
Answered: 2019-11-12
Question
I would like to test anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845 on human brain for research purposes, then I may be interested in using anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Mitchell
Verified customer
Asked: 2018-09-17
Answer
The products we sell, including anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-17
Question
Can you help my question with product M01845, anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jackson
Verified customer
Asked: 2016-07-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01845 anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-12
Question
Does anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845 work on canine IF with cajal-retzius cell fetal brain cortex?
B. Jackson
Verified customer
Asked: 2013-09-12
Answer
Our lab technicians have not validated anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal antibody M01845 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cajal-retzius cell fetal brain cortex in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-12
